Media (Isolation Procedure for Microorganisms) :.Isolation Procedure for Microorganisms :.However, the fungal isolates shall be used to verify the growth promotion ability of the media used for monitoring.In the case of fungi obtained from monitoring, these isolates may not be identified always.Allot the numbers of isolates as per the above procedure defined.The identified isolates shall be preserved for a period of one year using the seed lot technique and maintain in Annexure- 2.Verify the growth promotion ability of the media using the Isolate initially and record in Annexure- 3.In case any of the isolates are identified as Specified Microorganisms, notify the Quality Assurance and Production department.Evaluate the isolates, summarize the identification profile and the recommendations to the Quality Assurance through an addendum to the OOS document.Isolate and identify the colonies if the total microbial counts exceed the action limits (Out of Specification results) in case of the finished products.Isolates from the Non-Sterile Products (Oral Solid Dosage Forms) – (Isolation of Microorganisms):.YY- Last Two Digits of the current year.Allot the number of seed lots of Environment and Water isolates as below.The identified isolates shall be preserved for a period of one year using the seed lot technique and maintain the Annexure-2.Verify the growth promotion ability of the media using the isolates initially and record in Annexure- 3.In case this organism is already identified (repeat isolate), then the database shall be updated accordingly, but the culture need not be preserved.In this context, if an isolate is found morphologically different, the isolate shall be picked, and take a photograph of the colony for colony shape, elevation and Edge refer Annexure-4 (but not limited to) and identified. Due to the physiological stress on the isolates, the organisms inherently may show up slightly different morphology.The characterization of the predominant flora shall be useful in investigating the excursions.